Figure 4.

Serum immunoglobulins and anti-dsDNA antibodies from patients with lupus nephritis bind to annexin II. (A) IgG binding activity to annexin II assessed by ELISA in serum samples from patients with lupus nephritis during remission or active disease, patients with nonrenal lupus, or healthy controls. The values represent the mean OD for triplicate wells. The horizontal line in each group represents the mean value. (B) Serum annexin II-binding activity correlated with circulating anti-dsDNA antibody levels in lupus nephritis patients with active disease (●) or remission (○). The P values refer to pooled data. (C) Control IgG or anti-dsDNA antibodies from patients with lupus nephritis during remission or active disease or patients with nonrenal lupus were also tested for their binding to annexin II by ELISA. The values represent the mean OD for triplicate wells. The horizontal line in each group represents mean OD for annexin II binding. (D) Annexin II-binding activity in anti-dsDNA antibody preparations from lupus nephritis patients with active disease (●) or remission (○) correlated with levels of circulating anti-dsDNA antibodies. P values refer to pooled data. (E) Annexin II-binding activity in 14 paired serum samples from patients with lupus nephritis. (F) Annexin II-binding activity in 14 paired anti-dsDNA antibody preparations isolated from patients with lupus nephritis.