Figure 5.

Annexin II expression is increased in renal biopsies from patients with lupus nephritis and colocalizes with IgG deposition. (A) Representative images of IgG deposition and annexin II expression in healthy kidney (top row), lupus nephritis (second and third rows), or nonlupus glomerular diseases (rows 4 through 8). Lupus nephritis* (third row) shows representative images whereby anti-human annexin II antibody was incubated with recombinant human annexin II before use. Enlarged images of the Merge column are shown in the two columns on the right. The arrows depict colocalization of IgG and annexin II, visualized as yellow staining in the Merge column. GN, glomerulonephritis; FSGS, focal segmental glomerulosclerosis. (B) Glomerular staining score for annexin II in renal specimens. (C) Glomerular staining score for IgG deposition in renal specimens. The horizontal line in each group represents the mean staining score. **P < 0.001 compared with nonlupus glomerular diseases.