Figure 8.

Human polyclonal anti-dsDNA antibodies deposit in the glomeruli of NZBWF1/J mice and colocalize with annexin II expression. (A) Human polyclonal anti-dsDNA antibodies or normal human IgG (100 μg) were injected intraperitoneally into NZBWF1/J mice or BALB/c mice on 10 consecutive days. Kidney sections were snap frozen, and IgG/annexin colocalization was assessed by immunohistochemical staining. Representative images are presented at magnification, ×400. The enlargements of the boxed area show colocalization of anti-dsDNA antibodies and annexin II in glomeruli from NZBWF1/J mice but not in nonautoimmune mice. The arrows depict areas of colocalization. (B) Glomerular staining score for IgG deposition in renal specimens obtained from BALB/c and NZBWF1/J mice. The horizontal line in each group represents the mean staining score. **P < 0.001 for anti-dsDNA Ab injected into NZBWF1/J mice versus control IgG injected into NZBWF1/J or BALB/c mice or anti-DNA Ab injected into BALB/c mice. (C) Measurement of human IgG concentration (upper panel) and mouse anti-human IgG levels (expressed as OD) (lower panel) in serum samples from BALB/c or predisease NZBWF1/J mice after the last injection of either control human IgG or anti-dsDNA antibodies. Negative controls include sera from aged-matched BALB/c and NZBWF1/J mice without Ig injection (n = 6 for each group). The horizontal line in each group represents the mean level. **P < 0.001 for no treatment versus control IgG or anti-dsDNA Ab administration in NZBWF1/J mice; §§P < 0.001 for no treatment versus control IgG or anti-dsDNA Ab administration in BALB/c mice; *P < 0.01 for no treatment versus control IgG injection in NZBWF1/J mice; §P < 0.01 for no treatment versus control IgG injection in BALB/c mice.