Figure 3.

Acetazolamide treatment restores ENaC activity in CCDs from pendrin-null mice. Panel A shows the effect of benzamil on transepithelial voltage, Vt, in individual tubules from mice given aldosterone alone, aldosterone plus NaHCO₃, or aldosterone plus NaHCO₃ plus acetazolamide. Each tubule studied was obtained from a separate mouse. Panel B shows the difference in Vt measured in the presence (BENZ) and absence (CON-1) of benzamil. Values were compared between pendrin-null and wild-type mice after each treatment protocol using ANOVA.