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Raising HCO3− concentration on the apical or the basolateral side of mpkCCD monolayers increases ENaC subunit abundance. α, β, and γ ENaC subunit abundance was quantified by immunoblot when HCO3− concentration was varied on the apical or the basolateral side of mpkCCD monolayers by substituting HCO3− for methanesulfonate in equal concentrations. Band densities are shown in the lower panels. The upper panels display representative gels. *P < 0.05, ANOVA.
