Table 3.
Comparative results of quantitative reverse transcriptase–PCR for selected injury response, inflammation, and fibrosis-related genes in T12 biopsies
| Gene Product | Normal (n = 39) versus IF Alone (n = 28) | Pa | Normal (n = 39) versus IF+i (n = 16) | P | IF Alone (n = 28) versus IF+i (n = 16) | P |
|---|---|---|---|---|---|---|
| Pattern 1 | ||||||
| IL-6 | 1.65 | 0.01 | 1.69 | 0.02 | −1.01 | 0.96 |
| CD106 (VCAM-1) | 1.33 | 0.02 | 1.36 | 0.01 | 1.00 | 0.98 |
| CD62L (L-selectin) | 1.75 | 0.01 | 3.69 | 0.00001 | 1.83 | 0.01 |
| CD28 | 1.43 | 0.02 | 2.30 | 0.0001 | 1.47 | 0.04 |
| Pattern 2 | ||||||
| CD14 | 1.15 | 0.22 | 1.43 | 0.01 | 1.29 | 0.05 |
| IFN-γ | 1.10 | 0.77 | 2.60 | 0.001 | 2.18 | 0.04 |
| CXCL9 (MIG) | −1.26 | 0.53 | 2.59 | 0.01 | 3.47 | 0.0006 |
| CXCL10 (IP-10) | −1.02 | 0.93 | 1.90 | 0.03 | 1.91 | 0.02 |
| ubiquitin D (FAT10, diubiquitin) | 1.23 | 0.29 | 2.32 | 0.0007 | 1.69 | 0.02 |
| FOXP3 | 1.06 | 0.68 | 2.10 | 0.01 | 1.60 | 0.005 |
| Pattern 3 | ||||||
| TNF | 1.26 | 0.16 | 1.87 | 0.001 | 1.37 | 0.16 |
| TGF-β1 | 1.23 | 0.11 | 1.42 | 0.02 | 1.15 | 0.33 |
| TLR-4 | 1.21 | 0.06 | 1.44 | 0.02 | 1.27 | 0.20 |
| TLR-2 | 1.18 | 0.17 | 1.45 | 0.01 | 1.11 | 0.48 |
| Pattern 4 | ||||||
| CD74 | 1.10 | 0.31 | 1.23 | 0.13 | 1.04 | 0.75 |
| fibronectin 1 | 1.27 | 0.20 | 1.74 | 0.14 | 1.38 | 0.42 |
| collagen type IV α1 | 1.18 | 0.12 | 1.10 | 0.59 | −1.10 | 0.56 |
| Pattern 5 | ||||||
| VEGF | −1.07 | 0.52 | −1.36 | 0.02 | −1.29 | 0.05 |
| TOLLIP | −1.03 | 0.75 | −1.61 | 0.001 | −1.67 | 0.004 |
| BCL2 | 1.06 | 0.60 | −1.42 | 0.04 | −1.62 | 0.01 |
Data are expressed as fold change in RNA amount for second compared with first named group. TOLLIP, toll interacting protein; VEGF, vascular endothelial growth factor.
aP values based on t test for between-group comparisons.