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. 2010 Nov 19;404(1):56–69. doi: 10.1016/j.jmb.2010.08.008

Fig. 3.

Fig. 3

Activity of NAL variants isolated and purified from the E192X saturation mutagenesis library with (a) Neu5Ac (0.5 mM) and (a) DPAH (0.5 mM). Each assay was carried out as described in Materials and Methods and contained ∼ 0.03 mg of purified enzyme.