Skip to main content
. 2010 Nov 19;404(1):56–69. doi: 10.1016/j.jmb.2010.08.008

Fig. 6.

Fig. 6

Lineweaver–Burk plot of the steady-state enzyme kinetics of E192N reaction in the absence and in the presence of the inhibitor THB: (●) absence of THB; (○) 0.25 mM THB; (▼) 0.5 mM THB; (▽) 1 mM THB; (■) 1.5 mM THB; (□) 2 mM THB. The initial rates of reaction were measured using the standard enzyme assay, and kinetic parameters were determined by fitting all data to the rate equation for a single-substrate reaction in the presence of a competitive inhibitor to measure the Ki for THB.