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. 2010 Dec;21(12):2090–2098. doi: 10.1681/ASN.2010040438

Figure 4.

Figure 4.

Minocycline does not prevent the disruption of the BBB. Double immunostaining for MBP (A, D, and G) and IgG (B, E, and H). Merged images (C, F, and I). (A through C) noncorrected group. (D through F) MINO (−) group. (G through I) MINO (0,12,24) group. In the noncorrected group, no demyelinative lesions were observed (A), and IgG staining was not detected (B). In the MINO (−) group, severe demyelination was observed (D), and strong IgG staining was detected around the vessels in the demyelinative lesions (E and F). The demyelination observed in the MINO (0,12,24) group (G) was mild in comparison with that of the MINO (−) group (D), whereas IgG extravasation was observed in the MINO (0,12,24) group (H and I). The brains were removed 2 days after the rapid correction of hyponatremia. The scale bars show 20 μm. (J) BBB permeability was quantified 24 hours after correction. All of the values are expressed as the mean ± SEM (n = 5). #P < 0.05 compared with the noncorrected group.