Table 3.
Inference of selection on zebrafish VL genes
| Clone accession no. | Most similar germline VL | FR/CDR | Observed mutationsa |
PMb | |
|---|---|---|---|---|---|
| R | S | ||||
| EF222425 | Chr1-V1 | FR | 0 | 1 | 0.01236 |
| CDR | 2 | 1 | 0.07169 | ||
| EF222424 | Chr1-V4 | FR | 3 | 2 | 0.00805 |
| CDR | 6 | 1 | 0.00373 | ||
| EF222420 | Chr12-V2 | FR | 0 | 2 | 0.00003 |
| CDR | 10 | 1 | 0.00009 | ||
| EF222431 | Chr12-V2 | FR | 2 | 1 | 0.00112 |
| CDR | 12 | 0 | 0.00001 | ||
| EF222434 | Chr12-V5P | FR | 0 | 0 | 0.04010 |
| CDR | 2 | 1 | 0.03863 | ||
| EF222430 | Chr12-V9 | FR | 3 | 3 | 0.02144 |
| CDR | 5 | 1 | 0.08865 | ||
| EF222433 | Chr12-V9 | FR | 4 | 3 | 0.00330 |
| CDR | 10 | 3 | 0.00614 | ||
| EF222427 | Chr19-V1 | FR | 0 | 0 | 0.20319 |
| CDR | 1 | 0 | 0.08465 | ||
| DV593802 | Chr19-V1 | FR | 0 | 0 | 0.20060 |
| CDR | 2 | 0 | 0.08303 | ||
| EF222426 | Chr19-V2 | FR | 0 | 0 | 0.15765 |
| CDR | 2 | 0 | 0.05997 | ||
| EF222442 | Chr24-V2 | FR | 0 | 0 | 0.02731 |
| CDR | 5 | 0 | 0.00313 | ||
| EF222441 | Chr24-V2 | FR | 0 | 0 | 0.03714 |
| CDR | 1 | 1 | 0.81578 | ||
| EF222440 | Chr24-V5 | FR | 0 | 0 | 0.00408 |
| CDR | 8 | 5 | 0.00108 | ||
| DT318541 | Chr24-V6 | FR | 1 | 1 | 0.00084 |
| CDR | 8 | 3 | 0.00505 | ||
| EF222421 | Chr24-V8 | FR | 0 | 0 | 0.13431 |
| CDR | 2 | 0 | 0.04538 | ||
Statistically significant values in bold. PFR is selection to preserve FR and PCDR infers antigen selection of CDR variants.
a
Replacement (R) and silent (S) mutations from germline VL (over global alignment lengths reported in Table 1).
b
PM; multinomial probability calculated that excess (for CDR) or scarcity (for FR) of mutations occurred by chance.