Figure 5.

DOCP treatment differentially alters urinary Na⁺ and K⁺ retention in WT and HKα-null mice. (A) Urinary volume, (B) Na⁺, and (C) K⁺ retention were measured in WT, HKα₁^−/− and HKα_1,2^−/− mice from the day preceding treatment (control) and over an 8-day period of DOCP treatment. Urinary electrolyte retention (μEq) was calculated as the urinary excretion per day subtracted from dietary intake on that day. (D–F) Comparisons of control urine values versus only day 8 of DOCP treatment are shown. All data were analyzed by a two-way repeated-measure ANOVA with post hoc Student-Newman-Keuls test and are shown as mean ± SEM. P < 0.05 is considered significant. Comparison of genotype within day: ^asignificant difference from WT; ^bsignificant difference from HKα₁^−/− mice. Comparison of genotype over the time course: ^csignificant difference from WT; ^dsignificant difference from HKα₁^−/− mice. ^eSignificant difference from control within the same genotype. WT, n = 10; HKα₁^−/−, n = 5; HKα_1,2^−/−, n = 5.