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. 2000 Nov 1;106(9):1115–1126. doi: 10.1172/JCI9594

Figure 3.

Figure 3

Confocal images showing the time course of VP/forskolin–induced and SNP-induced (10 μM) translocation of AQP2 to the apical plasma membrane in collecting duct (inner stripe) principal cells. (a) Collecting duct stained 2 minutes after addition of VP/forskolin; (b) stained 10 minutes after addition of VP/forskolin; (c) stained 2 minutes after addition of SNP; (d) stained 10 minutes after addition of SNP. AQP2 is distributed intracellularly before agonist addition, but shows a progressive translocation to the apical cell surface during incubation with VP/forskolin and SNP (see Figure 4 for a quantification of the time course of agonist-induced AQP2 translocation). Some apical staining is already detectable 2 minutes after agonist addition, but a significant amount of intracellular staining is still present at this early time point (a, c). The maximum effect is seen after 10 minutes of treatment, when most of the staining is located on the apical membrane (b, d). Bar, 10 μm.