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. 2000 Nov 1;106(9):1127–1137. doi: 10.1172/JCI9914

Figure 4.

Figure 4

Cleavage of PARP, lamins, and B23 after TNF-α/AcD treatment is diminished in catB–/– hepatocytes. Hepatocytes from catB+/+ and catB–/– mice were incubated for 24 hours with medium lacking or containing TNF-α + AcD. Whole-cell lysates were then prepared as described in Methods. Aliquots containing 50 μg protein were subjected to SDS-PAGE on gradient gels containing 5–15% acrylamide, transferred to nitrocellulose, and sequentially blotted for PARP, lamins A and C, lamin B1, or B23. Cleavage of the substrates was detected by the loss of the bands corresponding to the molecular weight of the native protein, and, in the case of B23, by the appearance of a new band (arrow). GRP78 served as a control for protein loading.