Figure 6.

Caspase-8 activation after TNF-α/AcD treatment is reduced in catB^–/– cells. After cells were incubated in medium without (cont.) or with TNF-α + AcD for the indicated lengths of time, cytosolic fractions were prepared. Aliquots containing 40 μg protein were subjected to SDS-PAGE on gels containing 15% acrylamide, transferred to nitrocellulose, and immunoblotted for caspase-8. Processing of caspase-8 was detected by the appearance of the 18- to 20-kDa (p20) and 10-kDa (p10) active fragments. β-Actin served as a control for protein loading. Results are representative of three independent experiments.