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. 2010 Oct 20;85(1):357–365. doi: 10.1128/JVI.01694-10

FIG. 4.

FIG. 4.

Replication kinetics of rNY1682-WT, rNY1682-E158G, and rNY1682-E627K in various cell lines. Confluent monolayers of the various cell lines were inoculated with the rNY1682-WT (WT), rNY1682-E158G (E158G), and rNY1682-E627K (E627K) viruses. Culture supernatants were harvested from MDCK cells at 2, 12, 24, 48, and 72 h p.i. (A) and from human lung epithelial cells (Calu-3) (B) or mouse rectal epithelial carcinoma cells (CMT-93) (C) at 2, 12, 24, 48, 72, and 96 h p.i. Viral titers were determined by TCID50 assay using MDCK cells. Averages of triplicate experiments are shown, with error bars representing SD. *, statistical analysis indicated that the titers of rNY1682-E158G were significantly greater than those of rNY1682-WT at 24 to 96 h p.i. (P = 2 × 10−10; F test).