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Effect of transient expression of a VSV M mutant on Akt dephosphorylation. (A) BSR-T7/5 cells were transfected with liposomes only (mock) or with the plasmids pBS-M (M wt) and pBS-M-NCP12.1(M33,51A) (M-mut). Cell lysates were harvested and the levels of Akt phosphorylation and VSV M protein determined. (B) The level of Akt phosphorylation in panel A was determined by densitometry. The level of Akt phosphorylation in the liposome-treated lane (mock) was considered 100%.
