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. 2010 Oct 20;85(1):606–620. doi: 10.1128/JVI.00767-10

FIG. 4.

FIG. 4.

CHIKV infection of HF leads to accumulation of dsRNA and IRF3-dependent transcription via IPS1. (A) Indirect immunofluorescence showing dsRNA in CHIKV-infected HFs (MOI = 10) at 0, 2, 4, 6, and 8 h postinfection. (B) Immunoblot showing levels of IPS1, Ser398-phosphorylated IRF3, total IRF3, and GAPDH after treatment of HFs with NS or IPS1-directed siRNA in the presence or absence of CHIKV infection (MOI = 10) at 24 h postinfection. (C) Transcriptional induction of IFN-β, Viperin, and ISG56 as measured by qPCR after treatment of HFs with NS or IPS1-directed siRNA. The cells were either left uninfected or were infected with SeV (░⃞) or CHIKV (▪; MOI = 10), and total RNA was harvested at 24 h postinfection. Values presented are normalized to transcriptional induction in the presence of NS siRNA (set to 1) and are representative of experiments performed in duplicate.