FIG. 2.

Gag-specific CD8⁺ T-lymphocyte responses in TRIF^−/− and TLR3^−/− mice. C57BL/6 (WT), TRIF^−/−, and TLR3^−/− mice (n = 4 to 8 mice/group) were immunized with a single i.m. injection of 3 × 10⁸ vp of rAd5, rAd5HVR48, rAd26, or rAd35 expressing SIV Gag. Gag-specific cellular immune responses were assayed by D^b/AL11 tetramer binding assays at multiple time points following injection (A); IFN-γ ELISPOT assays in response to a Gag peptide pool, the CD8⁺ T-lymphocyte epitopes AL11 and KV9, and the CD4⁺ T-lymphocyte epitope DD13 (B); and IFN-γ ICS assays by CD8⁺ T lymphocytes in response to a Gag peptide pool performed 5 weeks postimmunization (C). The composite responses represent pooled data from all mice that received rAd vectors. Mean responses with standard errors are shown.