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. 2010 Oct 20;85(1):315–323. doi: 10.1128/JVI.01597-10

FIG. 4.

FIG. 4.

Gag-specific CD8+ T-lymphocyte responses in IL-1R−/− and IL-18R−/− mice. C57BL/6, IL-1R−/−, and IL-18R−/− mice (n = 4 mice/group) were immunized with a single i.m. injection of 3 × 108 vp of rAd5, rAd5HVR48, rAd26, or rAd35 expressing SIV Gag. Gag-specific cellular immune responses were assayed by Db/AL11 tetramer binding assays at multiple time points following injection (A); IFN-γ ELISPOT assays in response to a Gag peptide pool, the CD8+ T-lymphocyte epitopes AL11and KV9, and the CD4+ T-lymphocyte epitope DD13 (B); and IFN-γ ICS assays by CD8+ T lymphocytes in response to a Gag peptide pool (C). ELISPOT and ICS assays were performed 5 weeks after immunization. The composite responses represent pooled data from all mice that received rAd vectors. Mean responses with standard errors are shown.