TABLE 1.
Binding affinities for the binding of selected anti-Gag aptamers to DP6-Gag and two mutant Gag proteins, 8N-Gag and Δ16-99-Gaga
| Aptamer | Binding specificitya | Mean Kd (nM)b ± SD |
||
|---|---|---|---|---|
| DP6-Gag | 8N-Gag | Δ16-99-Gag | ||
| DP6-1 | MA | 178 ± 31 | ND | >956.4 |
| DP6-5 | Gag* | 161 ± 10.4 | 104 ± 15.5 | 121 ± 7.2 |
| DP6-7 | Gag* | 179 ± 12.2 | 337 ± 10.6 | 143 ± 10 |
| DP6-32 | Gag* | 203 ± 22 | 515 ± 50.7 | 238 ± 18 |
| DP6-19 | NC | 113 ± 9.9 | 209 ± 17.1 | 122 ± 22.2 |
| DP6-12 | MA, NC | 130 ± 9.3 | 342 ± 76.3 | 156 ± 33.2 |
| DP6-10 | Gag* | 144 ± 11.2 | 208 ± 28.8 | 72.4 ± 6.7 |
| DP6-21 | MA | 233 ± 32.2 | ND | >593.4 |
| DP6-22 | Gag* | 100 ± 3.4 | 177 ± 11.6 | 159 ± 9.5 |
| DP6-42 | MA | 91.3 ± 4.4 | ND | >494 |
| DP6-44 | Gag* | 82.5 ± 2.1 | 77.9 ± 22.8 | 41.9 ± 5.3 |
a
Aptamers that did not bind to any of the Gag component proteins tested—MA, CA, or NC—are indicated by an asterisk (*).
b
Binding affinities (Kd) were determined by filter binding. The specificities were determined by testing the percent binding of each selected aptamer to recombinant purified MA, CA, and NC proteins. ND, not detected.