FIG. 6.

Phospholipase C activation enhances ZEBOV-GP-mediated entry into Axl-dependent cells but not Axl-independent cells. (A) Ability of CPZ to inhibit dextran uptake. SNB19 cells were incubated with the indicated amounts of CPZ for 1 h. After 1 h, FITC-labeled dextran was added in the presence of the drug. Dextran-treated cells were washed after 1 h and analyzed by flow cytometry for uptake of dextran. (B to E) Ability of a PLC inhibitor, but not a PI3K inhibitor, to inhibit FIV-ZEBOV transduction of Axl-dependent cells. SNB19 cells (B and D) or Vero cells (C and E) were treated for 1 h with the indicated amounts of kinase inhibitor. Cells were then incubated with FIV pseudovirions (MOI, 0.005) in the continued presence of drug. The medium was changed after 6 h, and transduced cells were fixed and stained for β-Gal activity 48 h following transduction. Data represent the averages and standard errors of three experiments performed in triplicate. *, P < 0.05; **, P < 0.001.