FIG. 2.

P bodies are disrupted in cells with substantial viral gene expression. (A) HeLa cells were infected for 0, 2, 4, and 6 h with PV at an MOI of 20 or 0.1 for 4 h (right panels only) and fixed and processed for immunofluorescence with antibodies against Dcp1a and PV 2C protein. (B) Autoradiograph of proteins labeled with [³⁵S]methionine showing kinetics of translation shutoff and viral protein translation. (C) Immunofluorescence micrographs of 30 randomly selected cells stained for Rck/p54 were quantitated for the P body size and number per cell using NIH Image, and box plots showing medians, upper and lower quartiles and outliers, were graphed (**, P < 0.005). (D) Results were also plotted as a scatter plot showing average P body (PB) size /cell versus number of foci/cell. Black squares, 0 hpi; orange triangles, 2 hpi; red diamonds, 4 hpi.