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. 2010 Oct 27;85(1):178–188. doi: 10.1128/JVI.00998-10

FIG. 7.

FIG. 7.

Poly(I:C) suppresses the activity of HPV8 late and early promoters independently of the IRF binding site. (A) RTS3b cells were transfected with the HPV8 NCR luciferase construct (0.5 μg) and IRF-7Δ247-467 expression vectors (0.4 μg). The total amount of DNA was adjusted with empty pFlag-CMV2 control vector. The EGFP expression vector (0.25 μg) was cotransfected. After 6 h, the cells were stimulated with poly(I:C) (10 μg/ml) or medium as a control. After 24 h posttransfection, the luciferase activity was measured and normalized to the protein concentration of the respective luciferase extracts and the percentage of EGFP-positive cells. The normalized luciferase activity of the control transfection was set at 1. Shown are the values averaged from two transfections performed in triplicate ± SD. (B) RTS3b cells were transfected with either the pALuc-HPV8A reporter construct containing an intact IRF-BS (pALuc-HPV8A) or with IRF-BS deleted (pALuc-HPV8Adel) (left panel) or the pALuc-HPV8B reporter construct (right panel). After 6 h, the cells were stimulated with 10 μg/ml poly(I:C). Twenty-four hours posttransfection, luciferase activity was measured and normalized to the protein concentration. The normalized luciferase activity of the control transfection was set at 1. Shown are the values averaged from three transfections performed in triplicate ± SD.