FIG. 6.
Inhibition of cathepsin B attenuates Ad5-induced IL-1β release. (A) PMA-differentiated THP-1 cells were rested in serum-free medium for 2 h and pretreated with increasing concentrations of the cathepsin B inhibitor CA074me for 30 min before being infected with Ad5gfp (open) or medium alone (filled) for 6 h. (B) PMA-differentiated THP-1 cells were rested in serum-free medium for 2 h, primed with PAM3CSK4 for 2 h, and pretreated with increasing concentrations of CA074me for 30 min before being infected with Ad5gfp (open) or medium alone (filled) for 1 h. The release of IL-1β was quantified by ELISA. Data represent the means and standard errors from 3 replicates.