Skip to main content
. 2010 Oct 20;85(1):243–253. doi: 10.1128/JVI.01749-10

FIG. 2.

FIG. 2.

Downregulation of Snm1p level inhibits the accumulation of TBSV degRNA in yeast lacking Met22p bisphosphate-3′-nucleotidase. The met22Δ yeast strain shows decreased Xrn1p activity, facilitating the detection of endoribonucleolytically cleaved TBSV repRNA products. (A) Northern blot analysis for detection of plus strands of TBSV DI-72 repRNA and the newly formed degRNAs and recRNAs from met22Δ yeast strains. The accumulating repRNA, degRNA, and recRNA are shown with arrows. The numbers at the bottom of the panel show percentages of degRNA2 accumulation normalized to the level of DI-72 repRNA (100% in each sample). The various 5′-to-3′ sequences present in the repRNA and the generated recRNAs and degRNAs are shown on the right. The data were obtained from 24 independent yeast streaks. (B) Ethidium bromide-stained gel image showing partial processing of 5.8S rRNA in yeast with downregulated Snm1p. (C) Western blot image showing reduced expression level of p33 replication protein in yeast with downregulated Snm1p.