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. 2010 Oct 20;85(1):243–253. doi: 10.1128/JVI.01749-10

FIG. 3.

FIG. 3.

Increased accumulation of TBSV recRNAs in yeast overexpressing plasmid-borne NME1 RNA. (A) Northern blot analysis of total RNA samples obtained from the shown yeast strains overexpressing wt NME1 RNA from a plasmid. The accumulating repRNA, degRNA, and recRNAs are shown with arrows. The numbers at the bottom of the panel show percentages of degRNA2, recRNA1, and recRNA2 accumulation normalized to the level of DI-72 repRNA (100% in each sample). The various sequences present in the (+)repRNA and the newly formed recRNAs are shown schematically on the right. (B) Northern blot analysis of total RNA samples obtained from yeast strain BY4741, with a normal level of NME1 RNA expression from the chromosome (lane 1) or overexpressing wt NME1 RNA from a plasmid and from the chromosome (lanes 2 to 4).