FIG. 2.

(A) Schematics of plasmids used to generate nonspreading virus replicon particles (VRPs). Antigenomic S segments contain a perfect replacement of GPC by either GFP or LUC, and the GPC-expressing plasmids correspond to either authentic JUNV or the JUNV/LASV chimeric variants. (B) VeroE6 cells were infected with GFP-expressing VRPs. Infected cells were fixed at 4 dpi, and JUNV proteins were detected with anti-JUNV rabbit serum and anti-rabbit Alexa fluor 594. Fluorescent photomicrographs were taken using specific wavelength filters (green channel, GFP; red channel, JUNV proteins) and merged digitally. (C) VeroE6 cells were infected with LUC-expressing VRPs. Supernatants of infected cells were collected at 1, 2, 3, and 4 dpi, and the luciferase activity was measured with a Renilla luciferase assay system.