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. 2010 Oct 27;85(1):112–122. doi: 10.1128/JVI.01837-10

FIG. 4.

FIG. 4.

(A) Left, schematics of plasmids used to generate fully infectious viruses expressing authentic or chimeric GPC. Recombinant viruses were generated in BSRT7/5 cells and passaged twice onto VeroE6 cells. Right, viral titers were determined by standard plaque assay. (B) One-step growth curves were examined by infecting VeroE6 cells, collecting infectious supernatants at 24-h intervals, and determining viral titers in a TCID50 assay.