Figure 3.

In situ hybridization analyses of SMαA, SMMHC, and SM22α at 7 and 14 days after carotid injury. Mice were sacrificed 7 or 14 days after injury, and carotids were collected, fixed in 10% formalin, and processed for paraffin sectioning. Sections were treated as described in Methods, hybridized with ³⁵S-UTP labeled antisense riboprobes specific for SMαA (a–d), SMMHC (e–h), or SM22α (i–l) mRNA for 16 hours at 50°C, treated with RNaseA, and washed at 55°C in 1×SSC/0.1% SDS. Sections were then exposed to photographic emulsion for 3 weeks. Serial sections probed with sense riboprobes served as controls. There was little nonspecific binding of any sense probe (data not shown). Arrows denote the internal elastic lamina, and the arrowheads denote the external elastic lamina.