FIG. 2.

Verification of protein interactions by coimmunoprecipitation. 293T cells were transfected with equal amounts of HA and FLAG-tagged vectors, and total protein was harvested 48 h posttransfection. Lysates were subjected to immunoprecipitation with α-HA antibody. Precipitated proteins were separated by SDS-PAGE, and blots were probed with α-FLAG antibody to detect precipitation of binding partners. An immunoblot of the total cell lysate confirmed the expression of each tagged protein. α-Tubulin is shown as a loading control.