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Reduced IGFBP-3 profile in GHR–/– mouse serum and partial restoration by IGF-I treatment. (a) Western ligand blot of serum from 6-week-old wild-type, GHR–/–, and GHR–/– mice treated with IGF-I for 2 weeks (GHR–/– + IGF-I). (b) Densitometric analysis of IGF-binding activity; male and female results combined. AP < 0.001, BP < 0.01 vs. wild-type, and CP < 0.001, DP < 0.01 vs. GHR–/–.
