FIG. 6.

Regulation of PABP accumulation by 4E-BP1 phosphorylation in HCMV-infected cells. NHDFs stably expressing epitope-tagged (FH) WT or AA mutant 4E-BP1 were growth arrested by serum deprivation and either mock infected (0 h) or infected with HCMV (MOI = 5). After 48 h, total protein was isolated, fractionated by SDS-PAGE, and analyzed by immunoblotting with anti-4E-BP1 antibody which recognizes both endogenous and ectopically expressed (FH-4E-BP1) forms. Long and short exposures of the ectopically expressed 4E-BP1 protein are shown.