FIG. 11.

Localization of Atto647-Ad2-GFP-pV capsids near nuclear pore complexes. POM121-mCherry-transfected HER-911 cells were infected with Atto647-Ad2-GFP-pV and live imaged with spinning-disc confocal microscopy with the focus near the bottom of the nucleus at 60 min p.i. The left panel shows images recoded in the Atto647, GFP, and cherry channels. Merged images are shown in pseudocolors in the right panel, where red represents Atto647-labeled Ad2, green represents GFP-pV, and blue represents POM121-mCherry. White arrows point to triple-positive particles (red, green, blue), yellow arrows point to red and green double-positive particles, and magenta arrows point to red and blue double-positive particles.