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. 2010 Nov 3;85(1):481–496. doi: 10.1128/JVI.01571-10

FIG. 7.

FIG. 7.

R70 disassembly marker-positive capsids lack GFP-pV. (A) Atto647-labeled Ad2-GFP-pV particles were internalized into HER-911 cells for the indicated times, fixed, and stained with the rabbit antihexon R70 antibody and secondary anti-rabbit Alexa 594-conjugated antibody to detect preferentially disassembled capsids. The images in each row are from the same corresponding field. Downsized differential interference (DIC) and DAPI images are shown in columns 2 and 3, respectively. Scale bar, 10 μm. (B) Quantification of R70-positive capsids and overall R70 fluorescence; (C) GFP-pV in R70-positive and -negative capsids; (D) Atto647 intensity as a function of infection time; (E) GFP-pV/Atto647 fluorescence ratios at different times of infection demonstrate progressive loss of GFP-pV from the capsids.