TABLE 2.
Tissue titers for all mouse constructs infected with VV, VVΔE3L, and VVΔK3L
| Mouse construct | Dose (log10) | Organ | Titer (PFU/mg) at day postinfectiona |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| VV |
VVΔE3L |
VVΔK3L |
|||||||||
| 2 | 4 | 6 | 2 | 4 | 6 | 2 | 4 | 6 | |||
| Wild type | 6 | Lung | 6.1 | 6.5 | 5.9 | 3.7 | 2.7 | 2.6 | 5.1 | 6.0 | 5.1 |
| RNase L−/− | 5 | Lung | 4.9 | 5.6 | 4.8 | 2.4 | 2.3 | ND | 4.3 | 5.4 | 5.1 |
| PKR−/− | 5 | Lung | 4.8 | 5.7 | 5.6 | 3.9 | 3.6 | 1.8 | 4.1 | 5.4 | 5.6 |
| RNase L−/− PKR−/− | 4 | Lung | 5.4 | 5.7 | 6.1 | 3.5 | 3.7 | 2.6 | 2.2 | 4.2 | 4.2 |
| Wild type | 6 | Liver | 3.6 | 6.4 | 7.6 | ND | ND | ND | 0.9 | 0.9 | 0.9 |
| RNase L−/− | 5 | Liver | 2.9 | 5.4 | 6.3 | ND | ND | ND | 1.3 | 0.4 | 0.7 |
| PKR−/− | 5 | Liver | 2.8 | 5.5 | 6.9 | ND | ND | ND | 1.2 | 1.1 | 0.9 |
| RNase L−/− PKR−/− | 4 | Liver | 3.0 | 3.9 | 3.7 | ND | ND | ND | 1.3 | 1.1 | 2.3 |
| Wild type | 6 | Spleen | 4.5 | 5.9 | 6.0 | ND | ND | ND | 0.8 | 1.7 | 0.8 |
| RNase L−/− | 5 | Spleen | 4.7 | 5.8 | 5.9 | ND | ND | ND | 1.2 | 0.9 | 0.7 |
| PKR−/− | 5 | Spleen | 4.1 | 6.2 | 6.6 | ND | ND | ND | 1.2 | 1.6 | 1.8 |
| RNase L−/− PKR−/− | 4 | Spleen | 2.6 | 4.8 | 4.2 | ND | ND | ND | 1.3 | 1.2 | 2.4 |
| Wild type | 6 | Gonad | 3.7 | 5.1 | 7.3 | ND | ND | ND | 1.0 | 0.8 | 3.1 |
| RNase L−/− | 5 | Gonad | 3.9 | 5.0 | 7.4 | ND | ND | ND | 1.3 | 1.0 | 0.7 |
| PKR−/− | 5 | Gonad | 3.7 | 5.3 | 6.2 | ND | ND | ND | 1.3 | 1.9 | 2.6 |
| RNase L−/− PKR−/− | 4 | Gonad | 4.4 | 5.0 | 6.1 | ND | ND | ND | 1.3 | 1.1 | 1.1 |
| Wild type | 6 | Brain | 5.8 | 6.4 | 7.3 | ND | ND | ND | 1.3 | 3.1 | 3.7 |
| RNase L−/− | 5 | Brain | 3.6 | 6.8 | 6.7 | ND | ND | ND | 1.1 | 2.4 | 2.1 |
| PKR−/− | 5 | Brain | 4.2 | 6.6 | 7.2 | ND | ND | ND | 1.1 | 2.4 | 3.6 |
| RNase L−/− PKR−/− | 4 | Brain | 6.5 | 5.9 | 6.1 | ND | ND | ND | 1.3 | 1.4 | 2.7 |
a
Values are averages for ≥10 mice per condition. ND, no virus detected. The limit of detection for this assay is 0.2 PFU/mg.