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White light transmission image of pseudo-mixed biofilm (left) with a close-up of the scanned region (right). Darker regions are S. sanguinis stained with CV, while lighter regions are unstained S. mutans. Assignments in the scan: dark circles, S. mutans; light circles, S. sanguinis; x, spectral outlier (for example, due to the stain). Circle sizes represent laser focus. (Bar length = 10 μm.)
