Table 2.
Summary of antibodies used for immunohistochemistry. Abbreviations: M = monoclonal; P = polyclonal; APP = amyloid precursor protein; Aβ = amyloid beta; NF = neurofilament.
| Antibody | Epitope protein/Amino acids | Type | Dilution | Company |
|---|---|---|---|---|
| 22C11 | APP/60‐100 | M | 1:40 | Chemicon |
| Karen | APP/N terminal | P | 1:800 | * |
| 6F/3D | Aβ/8‐17 | M | 1:50 | Dako |
| 4G8 | Aβ/17‐24 | M | 1:1000 | * |
| 13335 | Aβ/1‐42 | P | 1:1000 | * |
| AMY33 | Aβ/1‐28 | M | 1:500 | Zymed |
| BCO5 | Aβ‐42 C terminal | M | 1:10000 | * |
| BACE | β‐secretase Asp1 | P | 1:500 | Alpha |
| PS‐1 | C‐terminal presenilin | P | 1:200 | Zymed |
| L1 | Kinesin‐L | M | 1:200 | Chemicon |
| N52 | NF‐H | M | 1:400 | Sigma |
| CD10 | Neprilysin | M | 1:50 | Novocastra |
| NEP | Neprilysin | P | 1:500 | Chemicon |
| OX42 | Macrophage | M | 1:100 | Serotec |
| Macrophage | macrophage inflammatory protein | P | 1:100 | Abcam |
| SMI‐94 | Mylin basic protein | M | 1:1000 | Covance |
*
Antibody obtained courtesy of Dr V.M.‐Y. Lee