Fig. 2.

Analysis of nutlin-3a characteristics in murine blood. A, nutlin-3a blood cell partitioning. Nutlin-3a was spiked into murine whole blood at various concentrations and incubated for 30 min at 37°C. In one aliquot, nutlin-3a was measured in whole blood, and in another aliquot nutlin-3a was measured in the plasma. B, nutlin-3a plasma protein binding. Nutlin-3a was spiked into murine plasma at various concentrations and incubated for 30 min at 37°C. Plasma protein binding was determined by equilibrium microdialysis and is expressed as the percentage of the total nutlin-3a plasma concentration that is unbound. Bars represent the mean, and error bars represent the S.D.