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A, outline of radioreceptor assay binding of TNFα to cTfRMAb-TNFR. A goat anti-mouse (GAM) IgG1 Fc was plated, which bound the Fc region of cTfRMAb-TNFR. The TNFR extracellular domain (ECD) region of the fusion protein then bound the 125I-TNFα, which was displaced by the addition of unlabeled TNFα. B, the saturable binding was analyzed by a nonlinear regression analysis to yield the concentration, Kd, that gave 50% inhibition of TNFα binding to cTfRMAb-TNFR.
