Fig. 1.

Structures of the chelators used in this study (A) and the effect of DpT- and BpT-based iron chelators on HIV-1 transcription (B and C) in CEM GFP cells. A, chemical structures of the iron chelators examined in this study, including members of the DpT series, BpT series, 2-(3-nitrobenzoyl) pyridine thiosemicarbazone (NBpT) series, di-2-pyridylketone thiobenzoyl hydrazone (PKTBH), and 2-benzoylpyridine thiobenzoyl hydrazone (BPTBH). B and C, inhibition of HIV-1 transcription in CEM-GFP cells. CEM-GFP cells were infected with adenovirus carrying Tat and then incubated with the indicated chelators for 24 h at 37°C. GFP fluorescence was measured in live cells. B and C, show different iron chelators that were separated into two graphs for clarity. The chelators enclosed in boxes were those with the lowest IC₅₀ values for inhibition of HIV-1 transcription. IC₅₀ values were determined using Prism 3 software as described under Materials and Methods. Results are means of three independent experiments.