TABLE 1.
Linkers between Subunits
Added amino acids are shown in bold.
| Concatamer Pair | Sequence of Linkers and Adjacent Subunits | Linker Length |
|
|---|---|---|---|
| Amino Acids (C′-tail + Linker + Amino Acids Added in Restriction Sites) | If Linker Were Extended Random Coil | ||
| Å | |||
| β2(AGS)6α4 | SAPSSKEG(AGS)6RAHA | 23 + 18 + 2 = 43 | 258 |
| α4(AGS)6β2 | WLAGMIQEGT(AGS)6TGTEER | 8 + 18 + 6 = 32 | 192 |
| α4(AGS)12β2 | WLAGMIQEGT(AGS)12TGTEER | 8 + 36 + 6 = 50 | 300 |
| β3(AGS)6α4 | WLHSYHSR(AGS)6RAHA | 11 + 18 + 2 = 31 | 186 |
| β3(AGS)12α4 | WLHSYHGT(AGS)12RAHA | 11 + 36 + 2 = 49 | 294 |
| β3(AGS)6α6 | WLHSYHGT(AGS)6TGCATE | 11 + 18 + 4 = 33 | 198 |
| β3(AGS)12α6 | WLHSYHGT(AGS)12CATE | 11 + 36 + 2 = 49 | 294 |
| α6(AGS)6β2 | GNTGKSGT(AGS)6TGTEER | 10 + 18 + 4 = 32 | 192 |
| α6(AGS)12β2 | GNTGKSGT(AGS)12TGTEER | 10 + 36 + 4 = 50 | 300 |