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. Author manuscript; available in PMC: 2011 Apr 28.
Published in final edited form as: N Engl J Med. 2010 Oct 28;363(18):1693–1703. doi: 10.1056/NEJMoa1006448

Figure 1. Diagnosis of an EML4-ALK–Positive Non–Small-Cell Lung Cancer in a Single Representative Patient.

Figure 1

Panel A shows the results of a break-apart fluorescence in situ hybridization assay of tumor cells from a patient with rearrangement of the gene encoding anaplastic lymphoma kinase (ALK). The green probe hybridizes to the region immediately 5′ to ALK, and the red probe to the 3′ region. The separation of red and green probe signals (arrows) indicates a chromosomal rearrangement involving ALK. Close apposition of red and green probe signals indicates an intact wild-type copy of ALK. The probe that was used was the Vysis LSI ALK Dual Color, Break Apart Rearrangement Probe (Abbott Molecular). Panel B shows a light micrograph of the same tumor, revealing adenocarcinoma (hematoxylin and eosin). Panel C shows a representative sequence electropherogram of a reverse-transcriptase–polymerase-chain-reaction assay of EML4-ALK. The sequence of a junction between EML4 exon 6b and ALK exon 20 is shown. Panel D shows immunohistochemical analysis of ALK protein expression in tumor cells (brown) but not in adjacent normal bronchial epithelium (diaminobenzidine).