Fig. 1.

Overview of the structures and synthesis of mono- and bis-(M)ANT-substituted nucleotides. Nucleotides differ from each other in terms of base, phosphate chain length, and substitution of the 2′- and 3′-O-ribosyl group. In mono-(M)ANT-substituted nucleotides, the substituent can isomerize spontaneously between the 2′ and 3′ positions (Suryanarayana et al., 2009). In bis-(M)ANT-substituted nucleotides, this isomerization cannot occur anymore. In a single step, nucleotides and diversified isatoic anhydrides are reacted to the corresponding acylated nucleotides.