TABLE 2.
Characteristics of unique spectra of tryptic peptides of human and rat mitochondrial GSTZ1 identified by ESI-QTOF with 95% probability (calculated by using Scaffold 2 software)
Underlined amino acids were identified by MS with modifications.
| Peptide Sequence | Modifications Identified by Spectrum | Mascot ion Score | Observed m/z | Actual Peptide Mass | Spectrum Charge | Actual Minus Calculated Peptide Mass |
|---|---|---|---|---|---|---|
| Human | ||||||
| 41 DGGQQFSK 48 | 56.8 | 433.64 | 865.27 | 2 | −0.12 | |
| 49 DFQALNPMK 57 | Oxidation (+16) | 43.2 | 540.12 | 1078.23 | 2 | −0.28 |
| 207 QPDTPTELRA 216 | Pyro-Glua (−17) | 49.0 | 555.71 | 1109.39 | 2 | −0.14 |
| 207 QPDTPTELRA 216 | 42.5 | 564.24 | 1126.46 | 2 | −0.10 | |
| Rat | ||||||
| 41 GGQQFSEEFQTLNPMK 57 | Oxidation (+16) | 37.9 | 657.87 | 1970.60 | 3 | −0.27 |
| 134 AITSGFNALEK 144 | 57.1 | 575.73 | 1149.44 | 2 | −0.16 |
a
Pyroglutamate (Pyro-Glu) formed from cyclization of N-terminal glutamine.