Fig. 7.

Additivity of SLP with conventional protection. Data are shown for recoveries of LVDP (A and C) and end-diastolic pressure (B and D). Mice were subjected to 5 days of morphine-induced SLP (or placebo), then isolated for ex vivo assessment of functional sensitivity to 25-min ischemia/45-min reperfusion. Isolated hearts were untreated or subjected to either acute 10 μM morphine (MOR) or IPC (three cycles × 1.5-min ischemia/2-min reperfusion) (A and B) or 50 μM adenosine (ADO), 10 μM 2-chloroadenosine (2CAD), or cardiac overexpression of A₁ adenosine receptors (A₁AR TG) (C and D). Data are means ± S.E.M. *, P < 0.05 versus corresponding placebo group; †, P < 0.05 versus SLP alone.