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. Author manuscript; available in PMC: 2011 Jan 3.
Published in final edited form as: Exp Eye Res. 2009 Jan 18;88(6):1020–1032. doi: 10.1016/j.exer.2009.01.002

Fig. 5.

Fig. 5

Silencing of Smad7 mRNA following transfection with specific Smad7 siRNA (siSmad7) or scrambled siRNA, and its effects on Smad7 and CTGF expression in untreated HTM cells (Co), or cells treated with BMP-7, TGF-β2 or combined BMP-7/TGF-β2. A. Real-time RT-PCR for Smad7 mRNA and Western blot analysis for Smad7. B. Real-time RT-PCR for Smad7 and CTGF mRNA in HTM cells, and Western blot analysis for CTGF in culture medium of HTM cells transfected with scrambled siRNA. C. Real-time RT-PCR for Smad7 and CTGF mRNA in HTM cells, and Western blot analysis for CTGF in culture medium of HTM cells transfected with siRNA against Smad7 (for real time RT-PCR experiments, means ± standard deviations of four independent experiments run in duplicate are shown, * p < 0.05, ** p < 0.01. The amounts of Smad7 mRNA were normalized to that of GAPDH).