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. Author manuscript; available in PMC: 2011 Oct 20.
Published in final edited form as: Bioconjug Chem. 2010 Oct 20;21(10):1912–1916. doi: 10.1021/bc100272z

Figure 2.

Figure 2

Viability of mammalian cells after Cu-ascorbate treatment ([CuSO4] and [THPTA] as indicated, [Na ascorbate] = 2.5 mM, DPBS, 4 °C, 5 minutes), followed by washing in buffer and incubation in growth media for 24 h. Cell viability was determined by CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, WI).(43) (B,C,E) Cells were grown for two days in the presence or absence of Ac4ManNAz, as indicated, before Cu treatment. Error bars indicate standard deviation from the averaging of results from at least three independent experiments.