Skip to main content
. Author manuscript; available in PMC: 2011 Oct 20.
Published in final edited form as: Bioconjug Chem. 2010 Oct 20;21(10):1912–1916. doi: 10.1021/bc100272z

Figure 3.

Figure 3

Representative labeling of cells (grown in the presence of 50 μM Ac4ManNAz for 48 h) with the indicated dye-alkynes (25 μM), in the presence of Cu catalyst (5 equiv. THPTA, 2.5 mM Na ascorbate, 1 mM aminoguanidine), in PBS pH 7.4 at 4 °C. The CuAAC reaction was conducted for 5 minutes unless otherwise noted, followed by washing in buffer and fixation for confocal microscopy imaging after incubation in fresh media for 15 minutes (except for panel D, for which the cells were incubated for 24 h before fixation and imaging). Nuclei were stained with DAPI (blue). Panels A, E, and I show the result of CuAAC labeling treatment with 1 on cells lacking the azido sugar, showing that non-specific adsorption or labeling with dye does not occur. The procedure for labeling of cells shown in panels J-L is described in the text. All images are 155 by 155 microns.