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. Author manuscript; available in PMC: 2011 Oct 20.
Published in final edited form as: Bioconjug Chem. 2010 Oct 20;21(10):1912–1916. doi: 10.1021/bc100272z

Figure 4.

Figure 4

Flow cytometry analysis of Jurkat cells, pre-incubated for 48 h in growth medium containing 10 μM Ac4ManNAz, and then labeled with 3 (50 μM) under standard conditions (CuSO4 and THPTA at the indicated concentrations, 2.5 mM Na ascorbate, 1 mM aminoguanidine, in DPBS, 4 °C, 5 min). (A) All samples were analyzed in triplicate; 10,000 events each were recorded in each case, and representative histograms are shown. (B) Mean fluorescence intensities with error bars representing standard deviations (statistical analysis performed using FlowJo software 8.7.1).