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Schematic diagram of the podocin-iCreERT2 construct. Human podocin promoter was inserted in the upstream of an iCre-ERT2 expression cassette, which also contained an intron derived from the rabbit β-globin and a bovine growth hormone poly A (bGHpA) sequence. PCR primers, iCre-PCR-F (forward) and iCre-PCR-R (reverse), were used to determine the presence of the iCre transgene in mice.
